CLC number:
On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
Crosschecked: 2022-12-15
Cited: 0
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Zhibiao BAI, Kai HU, Jiahuan YU, Yizhe SHEN, Chun CHEN. Macrophage migration inhibitory factor protects bone marrow mesenchymal stem cells from hypoxia/ischemia-induced apoptosis by regulating lncRNA MEG3[J]. Journal of Zhejiang University Science B, 2022, 23(12): 989-1001.
@article{title="Macrophage migration inhibitory factor protects bone marrow mesenchymal stem cells from hypoxia/ischemia-induced apoptosis by regulating lncRNA MEG3",
author="Zhibiao BAI, Kai HU, Jiahuan YU, Yizhe SHEN, Chun CHEN",
journal="Journal of Zhejiang University Science B",
volume="23",
number="12",
pages="989-1001",
year="2022",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B2200110"
}
%0 Journal Article
%T Macrophage migration inhibitory factor protects bone marrow mesenchymal stem cells from hypoxia/ischemia-induced apoptosis by regulating lncRNA MEG3
%A Zhibiao BAI
%A Kai HU
%A Jiahuan YU
%A Yizhe SHEN
%A Chun CHEN
%J Journal of Zhejiang University SCIENCE B
%V 23
%N 12
%P 989-1001
%@ 1673-1581
%D 2022
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2200110
TY - JOUR
T1 - Macrophage migration inhibitory factor protects bone marrow mesenchymal stem cells from hypoxia/ischemia-induced apoptosis by regulating lncRNA MEG3
A1 - Zhibiao BAI
A1 - Kai HU
A1 - Jiahuan YU
A1 - Yizhe SHEN
A1 - Chun CHEN
J0 - Journal of Zhejiang University Science B
VL - 23
IS - 12
SP - 989
EP - 1001
%@ 1673-1581
Y1 - 2022
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2200110
Abstract: ObjectiveThis research was performed to explore the effect of macrophage migration inhibitory factor (MIF) on the apoptosis of bone marrow mesenchymal stem cells (BMSCs) in ischemia and hypoxia environments.
MethodsThe cell viability of BMSCs incubated under hypoxia/ischemia (H/I) conditions with or without pretreatment with MIF or triglycidyl isocyanurate (TGIC) was detected using cell counting kit-8 (CCK-8) analysis. Plasmids containing long noncoding RNA (lncRNA) maternally expressed gene 3 (MEG3) or β;-Catenin small interfering RNA (siRNA) were used to overexpress or downregulate the corresponding gene, and the p53 signaling pathway was activated by pretreatment with TGIC. The influences of MIF, overexpression of lncRNA MEG3, activation of the p53 signaling pathway, and silencing of β;-Catenin on H/I-induced apoptosis of BMSCs were revealed by western blotting, flow cytometry, and terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick-end labeling (TUNEL) staining.
ResultsFrom the results of CCK-8 assay, western blotting, and flow cytometry, pretreatment with MIF significantly decreased the H/I-induced apoptosis of BMSCs. This effect was inhibited when lncRNA MEG3 was overexpressed by plasmids containing MEG3. The p53 signaling pathway was activated by TGIC, and β;-Catenin was silenced by siRNA. From western blot results, the expression levels of β;-Catenin in the nucleus and phosphorylated p53 (p-p53) were downregulated and upregulated, respectively, when the lncRNA MEG3 was overexpressed. Through flow cytometry, MIF was also shown to significantly alleviate the increased reactive oxygen species (ROS) level of BMSCs caused by H/I.
ConclusionsIn summary, we conclude that MIF protected BMSCs from H/I-induced apoptosis by downregulating the lncRNA MEG3/p53 signaling pathway, activating the Wnt/β;-Catenin signaling pathway, and decreasing ROS levels.
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