CLC number:
On-line Access: 2024-04-16
Received: 2023-09-10
Revision Accepted: 2024-02-22
Crosschecked: 0000-00-00
Cited: 0
Clicked: 213
Chunxiang Lu, Chuang Gao, Hao Qiao, Yi Zhang, Huazhen Liu, Aoxiang Jin, Yuanyuan Liu. Spheroid construction strategies and application in 3D bioprinting[J]. Journal of Zhejiang University Science D, 2024, 7(5): 800-818.
@article{title="Spheroid construction strategies and application in 3D bioprinting",
author="Chunxiang Lu, Chuang Gao, Hao Qiao, Yi Zhang, Huazhen Liu, Aoxiang Jin, Yuanyuan Liu",
journal="Journal of Zhejiang University Science D",
volume="7",
number="5",
pages="800-818",
year="2024",
publisher="Zhejiang University Press & Springer",
doi="10.1007/s42242-024-00273-7"
}
%0 Journal Article
%T Spheroid construction strategies and application in 3D bioprinting
%A Chunxiang Lu
%A Chuang Gao
%A Hao Qiao
%A Yi Zhang
%A Huazhen Liu
%A Aoxiang Jin
%A Yuanyuan Liu
%J Journal of Zhejiang University SCIENCE D
%V 7
%N 5
%P 800-818
%@ 1869-1951
%D 2024
%I Zhejiang University Press & Springer
%DOI 10.1007/s42242-024-00273-7
TY - JOUR
T1 - Spheroid construction strategies and application in 3D bioprinting
A1 - Chunxiang Lu
A1 - Chuang Gao
A1 - Hao Qiao
A1 - Yi Zhang
A1 - Huazhen Liu
A1 - Aoxiang Jin
A1 - Yuanyuan Liu
J0 - Journal of Zhejiang University Science D
VL - 7
IS - 5
SP - 800
EP - 818
%@ 1869-1951
Y1 - 2024
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1007/s42242-024-00273-7
Abstract: Tissue engineering has been striving toward designing and producing natural and functional human tissues. Cells are the
fundamental building blocks of tissues. Compared with traditional two-dimensional cultured cells, cell spheres are threedimensional (3D) structures that can naturally form complex cell–cell and cell–matrix interactions. This structure is close
to the natural environment of cells in living organisms. In addition to being used in disease modeling and drug screening,
spheroids have significant potential in tissue regeneration. The 3D bioprinting is an advanced biofabrication technique. It
accurately deposits bioinks into predesigned 3D shapes to create complex tissue structures. Although 3D bioprinting is
efficient, the time required for cells to develop into complex tissue structures can be lengthy. The 3D bioprinting of spheroids
significantly reduces the time required for their development into large tissues/organs during later cultivation stages by printing
them with high cell density. Combining spheroid fabrication and bioprinting technology should provide a new solution to
many problems in regenerative medicine. This paper systematically elaborates and analyzes the spheroid fabrication methods
and 3D bioprinting strategies by introducing spheroids as building blocks. Finally, we present the primary challenges faced
by spheroid fabrication and 3D bioprinting with future requirements and some recommendations.
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