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Abstract: The production of butyric acid by pHp?wd=%28Clostridium butyricum%29&ck%5B%5D=abstract&ck%5B%5D=keyword'>Clostridium butyricum ZJUCB at various pH values was investigated. In order to study the effect of pH on cell growth, butyric acid biosynthesis and reducing sugar consumption, different cultivation pH values ranging from 6.0 to 7.5 were evaluated in 5-L bioreactor. In controlled pH pHp?wd=%28batch fermentation%29&ck%5B%5D=abstract&ck%5B%5D=keyword'>batch fermentation, the optimum pH for cell growth and butyric acid production was 6.5 with a cell yield of 3.65 g/L and butyric acid yield of 12.25 g/L. Based on these results, this study then compared batch and fed-pHp?wd=%28batch fermentation%29&ck%5B%5D=abstract&ck%5B%5D=keyword'>batch fermentation of butyric acid production at pH 6.5. Maximum value (16.74 g/L) of butyric acid concentration was obtained in fed-pHp?wd=%28batch fermentation%29&ck%5B%5D=abstract&ck%5B%5D=keyword'>batch fermentation compared to 12.25 g/L in pHp?wd=%28batch fermentation%29&ck%5B%5D=abstract&ck%5B%5D=keyword'>batch fermentation. It was concluded that cultivation under fed-pHp?wd=%28batch fermentation%29&ck%5B%5D=abstract&ck%5B%5D=keyword'>batch fermentation mode could enhance butyric acid production significantly (P<0.01) by C. butyricum ZJUCB.

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