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Abstract: We identified a novel gene ST13 from a subtractive cDNA library of normal intestinal mucosa in 1993, more studies showed that ST13 was a co-chaperone of Hsp70s. Recently we detected the ST13 gene expression in tumor tissue and adjacent normal tissue of the same colorectal cancer patient and investigated if the ST13 gene expression might have any prognostic value. Analysis was performed at molecular level by reverse transcription-PCR using real-time detection method. We measured two genes simultaneously, ST13 as the target gene and glyceraldehydes-3-phosphate dehydrogenase as a reference gene, in primary colorectal tumor specimens and tumor-adjacent normal mucosa specimens from 50 colorectal cancer patients. The expression levels of the ST13 gene were significantly decreased in primary tumors compared with adjacent mucosa (P<0.05). But there were no significant differences in the expression of ST13 as compared with different Dukes’ stage, tumor differentiation grade, invasion depth, lymph node metastasis and disease-specific survival.

[1] Ballinger, C.A., Connell, P., Wu, Y., Hu, Z., Thompson, L.J., Yin, L.Y., Patterson, C., 1999. Identification of CHIP, a novel tetratricopeptide repeat-containing protein that interacts with heat shock proteins and negatively regulates chaperone functions. Mol. Cell Biol., 19(6):4535-4545.

[2] Bruce, B.D., Churchich, J., 1997. Characterization of the molecular-chaperone function of the heat-shock-cognate-70-interacting protein. Eur. J. Biochem., 245:738-744.

[3] Bukau, B., Horwich, A.L., 1998. The Hsp70 and Hsp60 chaperone machines. Cell, 92:351-366.

[4] Dundas, S.R., Lawrie, L.C., Rooney, P.H., Murray, J.I., 2005. Mortalin is over-expressed by colorectal adenocarcinomas and correlates with poor survival. J. Pathol., 205(1):74-81.

[5] Fan, J.H., Yang, W., Sai, J., Richmond, A., 2002. Hsc/Hsp70 interacting protein (Hip) associates with CXCR2 and regulates the receptor signaling and trafficking. J. Bio. Chem., 277(8):6590-6597.

[6] Gelmini, S., Orlando, C., Sestini, R., Vona, J., Pinzani, P., Giacca, M., Pazzagali, M., 1997. Quantitative polymerase chain reaction-based homogeneous assay with fluorogenic probe to measure c-erB-2 oncogene amplification. Clinical Chemistry, 43(5):752-758.

[7] Hartl, F.U., 1996. Molecular chaperones in cellular protein folding. Nature, 381:571-580.

[8] Hohfeld, J., Jentsch, S., 1997. GrpE-like regulation of the Hsc70 chaperone by the anti-apoptotic protein BAG-1. EMBO J., 16:6209-6216.

[9] Hohfeld, J., Minami, Y., Hartl, F.U., 1995. Hip, a novel cochaperone involved in the eukaryotic Hsc70/Hsp40 reaction cycle. Cell, 83:589-598.

[10] Irmen, H., Hohfeld, J., 1997. Characterization of functional domains of the eukaryotic co-chaperone Hip. J. Bio. Chem., 272(4):2230-2235.

[11] Jemal, A., Tiwari, R.C., Murray, T., Ghafoor, A., Samuels, A., Ward, E., Feuer, E.J., Thun, M.J., 2004. American cancer society. Cancer statistics. CA Cancer J. Clin., 54(1):8-29.

[12] Kikuchi, R., Noguchi, T., Takeno, S., Funada, Y., Moriyama, H., Uchida, Y., 2002. Nuclear BAG-1 expression reflects malignant potential in colorectal carcinomas. Br. J. Cancer, 87(10):1136-1139.

[13] Lazaris, A.C., Theodoropoulos, G.E., Davaris, P.S., Panoussopoulos, D., Nakopoulous, Z., Ittas, C., Golematis, B.C., 1995. Heat shock protein 70 and HLA-DR molecules tissue expression. Prognostic implications in colorectal cancer. Dis. Colon. Rectum., 38(7):739-745.

[14] Mo, Y.Q., Zheng, S., Shen, D.J., 1996. Differential expression of HSU17714 gene in colorectal cancer and normal colonic mucosa. Chinese Journal of Oncology, 18(4):241-243 (in Chinese).

[15] Nollen, E.A., Kabakov, A.E., Brunsting, J.F., Kanon, B., Hohfeld, J., Kampinga, H.H., 2001. Modulation of in vivo Hsp70 chaperone activity by Hip and BAG-1. J. Biol. Chem., 276:4677-4682.

[16] Nylandsted, J., Rohde, M., Brand, K., Bastholm, L., Elling, F., Jaattela, M., 2000. Selective depletion of heat shock protein 70 (Hsp70) activates a tumor-specific death program that is independent of caspase and bypass of Bcl-2. Proc. Natl. Aca. Sci. USA, 97:7871-7876.

[17] Prapapanich, V., Chen, S., Nair, S.C., Rimerman, R.A., Smith, D.F., 1996a. Molecular cloning of human P48, a transient component of progesterone receptor complexes and an Hsp70-binding protein. Molecular Endocrinology, 10(4):420-431.

[18] Prapapanich, V., Chen, S., Toran, E.G., Rimerman, R.A., Smith, D.F., 1996b. Mutational analysis of the hsp70-interacting protein Hip. Molecular and Cellular Biology, 16(1):6200-6207.

[19] Ravagnan, L., Gurbuxani, S., Susin, S.A., Maisse, C., Daugas, E., Zamzami, N., Mak, T., Jaattela, M., Penninger, J.M., Garrido, C., Kroemer, G., 2001. Heat-shock protein 70 antagonizes apoptosis-inducing factor. Nature-Cell Biol., 3:839-843.

[20] Swan, D.C., Tucker, R.A., Holloway, B.P., Icenogle, J.P., 1997. A sensitive, type-specific, fluorogenic probe assay for detection of human papillomavirus DNA. J. Clin. Microbiol., 35(4):886-891.

[21] Vogelstein, B., Fearon, E.R., 1988. Genetic alterations during colorectal tumor development. N. Engl. J. Med., 312(9):525.

[22] Ye, F., Fang, S.C., Zheng, S., 2001. In situ analysis the expression of new ST13 gene in colorectal cancer and its prognostic value. Journal of Practical Oncology, 16(5):318-321 (in Chinese).

[23] Zheng, S., 1993. Application of subtractive hybridization in screening for colorectal cancer-related genes. Chin. Med. J., 8:100.

[24] Zheng, S., 1997. Recent study on colorectal cancer in China: early detection and novel related gene. Chin. Med. J., 110(4):309-310.

[25] Zheng, S., Cai, X.H., Cao, J., Geng, L.Y., 1997. Screening and identification of down-regulated genes in colorectal carcinoma by subtractive hybridization: a method to identify putative tumor suppressor genes. Chin. Med. J., 110(7):543-549.