CLC number: R54
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Received: 2005-02-23
Revision Accepted: 2005-06-28
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YANG Yun-mei, LV Xue-ying, HUANG Wei-dong, XU Zhe-rong, WU Ling-jiao. Study of androgen and atherosclerosis in old-age male[J]. Journal of Zhejiang University Science B, 2005, 6(9): 931-935.
@article{title="Study of androgen and atherosclerosis in old-age male",
author="YANG Yun-mei, LV Xue-ying, HUANG Wei-dong, XU Zhe-rong, WU Ling-jiao",
journal="Journal of Zhejiang University Science B",
volume="6",
number="9",
pages="931-935",
year="2005",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2005.B0931"
}
%0 Journal Article
%T Study of androgen and atherosclerosis in old-age male
%A YANG Yun-mei
%A LV Xue-ying
%A HUANG Wei-dong
%A XU Zhe-rong
%A WU Ling-jiao
%J Journal of Zhejiang University SCIENCE B
%V 6
%N 9
%P 931-935
%@ 1673-1581
%D 2005
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2005.B0931
TY - JOUR
T1 - Study of androgen and atherosclerosis in old-age male
A1 - YANG Yun-mei
A1 - LV Xue-ying
A1 - HUANG Wei-dong
A1 - XU Zhe-rong
A1 - WU Ling-jiao
J0 - Journal of Zhejiang University Science B
VL - 6
IS - 9
SP - 931
EP - 935
%@ 1673-1581
Y1 - 2005
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2005.B0931
Abstract: Objective: To observe the difference of androgen and inflammatory cytokines level in atherosclerosis and analyse their relations. Method: Both carotid arteries and arteries of lower extremity were subjected to ultrasonic examination by Doppler’s method. Those with much atheromatous plaque formation were ranged into case group, and those with normal result formed control group. Total, free testosterone and estradiol were assayed by radioimmunoassay. C reactive protein (CRP) was assayed by nepheloturbidity. Tumor necrosis factor-α (TNF-α), Interleukin-6 (IL-6), Interleukin-8 (IL-8), Interleukin-10 (IL-10), Interleukin-18 (IL-18), soluble intercellular adhesion molecule-1 (sICAM-1) and soluble vascular cell adhesion molecule-1 (sVCAM-1) were assayed by ELISA. The mean difference between two groups and the correlation between free testosterone and cytokines were analysed. Results: free testosterone was (6.337±3.371) pg/L in case group and (11.375±4.733) pg/L in control group, P<0.01. No differences were found in total testosterone and estradiol. CRP was (27.294±10.238) mg/L in case group and (12.843±6.318) mg/L in control group, P<0.01. IL-6 was (41.700±31.385) pg/L in case group and (25.396±20.772) pg/L in control group, P<0.05. IL-8 was (89.249±58.357) pg/L in case group and (67.873±31.227) pg/L in control group, P<0.05. sICAM-1 was (470.491±134.078) pg/L in case group and (368.487±97.183) pg/L in control group, P<0.01. sVCAM-1 was (537.808±213.172) pg/L in case group and (457.275±157.273) pg/L in control group, P<0.05. There were no differences in TNF-α, IL-10 and IL-18. Correlation analysis showed that FT (free testosterone) had negative correlation with CRP, IL-6 and sICAM-1. Among them FT had well correlation with CRP, correlation index was −0.678. Conclusion: free testosterone was in negative correlation with atherosclerosis in old-age male. free testosterone may have the role of anti-atherosclerosis, and this effect was not achieved by its transformation to estradiol. Low free testosterone level was followed by increased level of inflammatory cytokines. Low free testosterones coexist with inflammation and they both affect the process of atherosclerosis in old-age male.
[1] Blake, G.J., Ridker, P.M., 2001. Novel clinical markers of vascular wall inflammation. Cire. Res., 89:763-771.
[2] Chen, Y.H., Lin, S.J., Ku, H.H., Shiao, M.S., Lin, F.Y., Chen, J.W., Chen, Y.L., 2001. Salvianolic acid B attenuates VCAM-1 and ICAM-1 expression in TNF-alpha-treated human aortic endothelial cells. J. Cell Biochem., 82(3):512-521.
[3] de Pergola, G., Pannacciulli, N., Ciccone, M., Tartagni, M., Rizzon, P., Giorgino, R., 2003. Free testosterone plasma levels are negatively associated with the intima-media thickness of the common carotid artery in overweight and obese glucose-tolerant young adult men. Int. J. Obes. Relat. Metab. Disord., 27(7):803-807.
[4] English, K.M., Steeds, R.P., Jones, T.H., Diver, M.J., Channer, K.S., 2000a. Low-dose transdermal testosterone therapy improves angina threshold in men with chronic stable angina: A randomized, double-blind, placebo-controlled study. Circulation, 102:1906-1911.
[5] English, K.M., Mandour, O., Steeds, R.P., Diver, M.J., Jones, T.H., Channer, K.S., 2000b. Men with coronary artery disease have lower levels of androgens than men with normal coronary angiograms. Eur. Heart J., 21:890-894.
[6] George, J., Greenberg, S., Barshack, I., Goldberg, I., Keren, G., Roth, A., 2003. Immunity to heart shock protein 65−an additional determinant in intima thickening. Atherosclerosis, 168(1):33-38.
[7] Giuseppe, M.C., Leonardo, F., Pagnotta, P., Pelliccia, F., Panina, G., Cerquetani, E., Lilla della Monica, P., Bonfigli, B., Volpe, M., Chierchia, S.L., 1999. Acute anti-ischemic effect of testosterone in men with coronary artery disease. Circulation, 99:1666-1670.
[8] Guan, Y., Zhang, M.X., Yuan, H., 1998. The study of relationship among coronary heart disease, serum levels of TNF and IL-6. J. Clin. Cardiol., 14(6):325-327 (in Chinese).
[9] Huittinen, T., Leinonen, M., Tenkanen, L., Virkkunen, H., Manttari, M., Palosuo, T., Manninen, V., Saikku, P., 2003. Synergistic effect of persistent chlamydia pneumoniae infection, autoimmunity and inflammation on coronary risk. Circulation, 107(20):2566-2570.
[10] Li, J.J., 2004. Silent myocardial ischemia may be related to inflammatory response. Med. Hypotheses, 62:252-256.
[11] Lv, H.S., Wu, S.Z., 2001. Caducity and the change of androgen level. J. First Mil. Med. Univ., 21(5):387-389 (in Chinese).
[12] Makinen, J., Jarvisalo, M.J., Pollanen, P., Perheentupa, A., Irjala, K., Koskenvuo, M., Huhtaniemi, I., Raitakari, O.T., 2005. Increased carotid atherosclerosis in andropausal middle-aged men. J. Am. Coll. Cardiol., 45(10):1603-1608.
[13] Malkin, C.J., Pugh, P.J., Jones, R.D., Kapoor, D., Channer, K.S., Jones, T.H., 2004. The effect of testosterone replacement on endogenous inflammatory cytokines and lipid profiles in hypogonadal men. J. Clin. Endocrinol. Metab., 89(7):3313-3318.
[14] Mallat, Z., Henry, P., Fressonnet, R., Alouani, S., Scoazec, A., Beaufils, P., Chvatchko, Y., Tedgui, A., 2002. Increased plasma concentrations of interleukin-18 in acute coronary syndromes. Heart, 88(5):467-469.
[15] Ross, R., 1999. Ateriosclerosis-an inflammation disease. N. Engl. J. Med., 340:115-126.
[16] Ross, R., Glomset, J., Kariya, B., Harker, L., 1974. A platelet-dependent serum factor that stimulates the proliferation of arterial smooth muscle cells in vitro. Proc. Natl. Acad. Sci. USA, 71(4):1207-1210.
[17] Signorelli, S.S., Mazzarino, M.C., Di Pino, L., Malaponte, G., Porto, C., Pennisi, G., Marchese, G., Costa, M.P., Digrandi, D., Celotta, G., Virgilio, V., 2003. High circulating levels of cytokines (IL-6 and TNFα), adhesion molecules (VCAM-1 and ICAM-1) and selectins in patients with peripheral arterial disease at rest and after a treadmill test. Vasc. Med., 8(1):15-19.
[18] Somjen, D., Kohen, F., Jaffe, A., Amir-Zaltsman, Y., Knoll, E., Stern, N., 1998. Effects of gonadal steroids and their antagonists on DNA synthesis in human vascular cells. Hypertension, 32(1):39-45.
[19] Waehre, T., Halvorsen, B., Damas, J.K., Yndestad, A., Brosstad, F., Gullestad, L., Kjekshus, J., Froland, S.S., Aukrust, P., 2002. Inflammatory imbalance between IL-10 and TNFα in unstable angina potential plaque stabilizing effects of IL-10. Eur. J. Clin. Invest., 32(11):803-810.
[20] Webb, C.M., Adamson, D.L., Zeigler, D., Collins, P., 1999. Effect of acute testosterone on myocardial ischemia in men with coronary artery disease. Am. J. Cardiol., 83:437-439.
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