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Journal of Zhejiang University SCIENCE B 2009 Vol.10 No.6 P.411-421


Epigallocatechin-3-gallate affects the growth of LNCaP cells via membrane fluidity and distribution of cellular zinc

Author(s):  Jun-guo YANG, Hai-ning YU, Shi-li SUN, Lan-cui ZHANG, Guo-qing HE, Undurti N. DAS, Hui RUAN, Sheng-rong SHEN

Affiliation(s):  Department of Food Science and Nutrition, Zhejiang University, Hangzhou 310029, China; more

Corresponding email(s):   shrshen@zju.edu.cn

Key Words:  Epigallocatechin-3-gallate (EGCG), Zinc, LNCaP, Membrane fluidity

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Jun-guo YANG, Hai-ning YU, Shi-li SUN, Lan-cui ZHANG, Guo-qing HE, Undurti N. DAS, Hui RUAN, Sheng-rong SHEN. Epigallocatechin-3-gallate affects the growth of LNCaP cells via membrane fluidity and distribution of cellular zinc[J]. Journal of Zhejiang University Science B, 2009, 10(6): 411-421.

@article{title="Epigallocatechin-3-gallate affects the growth of LNCaP cells via membrane fluidity and distribution of cellular zinc",
author="Jun-guo YANG, Hai-ning YU, Shi-li SUN, Lan-cui ZHANG, Guo-qing HE, Undurti N. DAS, Hui RUAN, Sheng-rong SHEN",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Epigallocatechin-3-gallate affects the growth of LNCaP cells via membrane fluidity and distribution of cellular zinc
%A Jun-guo YANG
%A Hai-ning YU
%A Shi-li SUN
%A Lan-cui ZHANG
%A Guo-qing HE
%A Undurti N. DAS
%A Sheng-rong SHEN
%J Journal of Zhejiang University SCIENCE B
%V 10
%N 6
%P 411-421
%@ 1673-1581
%D 2009
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0820400

T1 - Epigallocatechin-3-gallate affects the growth of LNCaP cells via membrane fluidity and distribution of cellular zinc
A1 - Jun-guo YANG
A1 - Hai-ning YU
A1 - Shi-li SUN
A1 - Lan-cui ZHANG
A1 - Guo-qing HE
A1 - Undurti N. DAS
A1 - Hui RUAN
A1 - Sheng-rong SHEN
J0 - Journal of Zhejiang University Science B
VL - 10
IS - 6
SP - 411
EP - 421
%@ 1673-1581
Y1 - 2009
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0820400

Objective: To evaluate effects of epigallocatechin-3-gallate (EGCG) on the viability, membrane properties, and zinc distribution, with and without the presence of Zn2+, in human prostate carcinoma LNCaP cells. Methods: We examined changes in cellular morphology and membrane fluidity of LNCaP cells, distribution of cellular zinc, and the incorporated portion of EGCG after treatments with EGCG, Zn2+, and EGCG+Zn2+. Results: We observed an alteration in cellular morphology and a decrease in membrane fluidity of LNCaP cells after treatment with EGCG or Zn2+. The proportion of EGCG incorporated into liposomes treated with the mixture of EGCG and Zn2+ at the ratio of 1:1 was 90.57%, which was significantly higher than that treated with EGCG alone (30.33%). Electron spin resonance (ESR) studies and determination of fatty acids showed that the effects of EGCG on the membrane fluidity of LNCaP were decreased by Zn2+. EGCG accelerated the accumulation of zinc in the mitochondria and cytosol as observed by atomic absorption spectrometer. Conclusion: These results show that EGCG interacted with cell membrane, decreased the membrane fluidity of LNCaP cells, and accelerated zinc accumulation in the mitochondria and cytosol, which could be the mechanism by which EGCG inhibits proliferation of LNCaP cells. In addition, high concentrations of Zn2+ could attenuate the actions elicited by EGCG.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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