CLC number:
On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
Crosschecked: 2023-07-17
Cited: 0
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Tao BAO, Naymul KARIM, Huihui KE, Jitbanjong TANGPONG, Wei CHEN. Polysaccharide isolated from wax apple suppresses ethyl carbamate-induced oxidative damage in human hepatocytes[J]. Journal of Zhejiang University Science B, 2023, 24(7): 574-586.
@article{title="Polysaccharide isolated from wax apple suppresses ethyl carbamate-induced oxidative damage in human hepatocytes",
author="Tao BAO, Naymul KARIM, Huihui KE, Jitbanjong TANGPONG, Wei CHEN",
journal="Journal of Zhejiang University Science B",
volume="24",
number="7",
pages="574-586",
year="2023",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B2200629"
}
%0 Journal Article
%T Polysaccharide isolated from wax apple suppresses ethyl carbamate-induced oxidative damage in human hepatocytes
%A Tao BAO
%A Naymul KARIM
%A Huihui KE
%A Jitbanjong TANGPONG
%A Wei CHEN
%J Journal of Zhejiang University SCIENCE B
%V 24
%N 7
%P 574-586
%@ 1673-1581
%D 2023
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2200629
TY - JOUR
T1 - Polysaccharide isolated from wax apple suppresses ethyl carbamate-induced oxidative damage in human hepatocytes
A1 - Tao BAO
A1 - Naymul KARIM
A1 - Huihui KE
A1 - Jitbanjong TANGPONG
A1 - Wei CHEN
J0 - Journal of Zhejiang University Science B
VL - 24
IS - 7
SP - 574
EP - 586
%@ 1673-1581
Y1 - 2023
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2200629
Abstract: Wax apple (Syzygium samarangense) has received growing research interest for its high nutritional and medicinal value due to its constituents such as polysaccharide, organic acids, flavonoids, minerals, and other substances. In this study, wax apple polysaccharide (WAP) was isolated from this plant and its protective effect against ethyl carbamate (EC)-induced oxidative damage was evaluated in human hepatocytes (L02 cells). Firstly, a series of analyses such as high-performance liquid chromatography (HPLC), high-performance gel permeation chromatography (HPGPC), Fourier transform infrared spectroscopy (FT-IR), gas chromatography/mass spectrometry (GC/MS), and 1H and 13C nuclear magnetic resonance (NMR) were conducted to identify the structure of WAP. Thereafter, in vitro cell experiments were performed to verify the protective effects of WAP against EC-induced cytotoxicity, genotoxicity, and oxidative damage in L02 cells. Our results revealed that WAP is composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, arabinose, and fucose in a molar ratio of 2.20:3.94:4.45:8.56:8.86:30.82:39.78:1.48. Using a combination of methylation and NMR spectroscopic analysis, the primary structure of WAP was identified as Araf-(1→, Glcp-(1→, →2)-Araf-(1→, →3)-Galp-(1→, →3)-Araf-(1→, and →6)-Galp-(1→. Cell experiments indicated that WAP exhibited significant protective effects on EC-treated L02 cells via suppressing cytotoxicity and genotoxicity, reducing reactive oxygen species (ROS) and O2•- formation, as well as improving mitochondrial membrane potential (MMP) and glutathione (GSH). In a nutshell, WAP has the potential as an important therapeutic agent or supplement for hepatic oxidative damage. Meanwhile, further studies are needed to prove the above effects in vivo at the biological and clinical levels.
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