Full Text:   <3548>

Summary:  <1727>

CLC number: R587.1

On-line Access: 2018-07-04

Received: 2017-05-13

Revision Accepted: 2017-07-03

Crosschecked: 2018-05-14

Cited: 0

Clicked: 5559

Citations:  Bibtex RefMan EndNote GB/T7714


Feng Zhou


-   Go to

Article info.
Open peer comments

Journal of Zhejiang University SCIENCE B 2018 Vol.19 No.7 P.559-569


Stability of a type 2 diabetes rat model induced by high-fat diet feeding with low-dose streptozotocin injection

Author(s):  Xiao-xuan Guo, Yong Wang, Kai Wang, Bao-ping Ji, Feng Zhou

Affiliation(s):  Beijing Key Laboratory of Functional Food from Plant Resources, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China; more

Corresponding email(s):   zf@cau.edu.cn

Key Words:  High-fat diet, Stability, Streptozotocin, Type 2 diabetes mellitus

Xiao-xuan Guo, Yong Wang, Kai Wang, Bao-ping Ji, Feng Zhou. Stability of a type 2 diabetes rat model induced by high-fat diet feeding with low-dose streptozotocin injection[J]. Journal of Zhejiang University Science B, 2018, 19(7): 559-569.

@article{title="Stability of a type 2 diabetes rat model induced by high-fat diet feeding with low-dose streptozotocin injection",
author="Xiao-xuan Guo, Yong Wang, Kai Wang, Bao-ping Ji, Feng Zhou",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Stability of a type 2 diabetes rat model induced by high-fat diet feeding with low-dose streptozotocin injection
%A Xiao-xuan Guo
%A Yong Wang
%A Kai Wang
%A Bao-ping Ji
%A Feng Zhou
%J Journal of Zhejiang University SCIENCE B
%V 19
%N 7
%P 559-569
%@ 1673-1581
%D 2018
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1700254

T1 - Stability of a type 2 diabetes rat model induced by high-fat diet feeding with low-dose streptozotocin injection
A1 - Xiao-xuan Guo
A1 - Yong Wang
A1 - Kai Wang
A1 - Bao-ping Ji
A1 - Feng Zhou
J0 - Journal of Zhejiang University Science B
VL - 19
IS - 7
SP - 559
EP - 569
%@ 1673-1581
Y1 - 2018
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1700254

Objective: The present study aims at determining the stability of a popular type 2 diabetes rat model induced by a high-fat diet combined with a low-dose streptozotocin injection. Methods: Wistar rats were fed with a high-fat diet for 8 weeks followed by a one-time injection of 25 or 35 mg/kg streptozotocin to induce type 2 diabetes. Then the diabetic rats were fed with regular diet/high-fat diet for 4 weeks. Changes in biochemical parameters were monitored during the 4 weeks. Results: All the rats developed more severe dyslipidemia and hepatic dysfunction after streptozotocin injection. The features of 35 mg/kg streptozotocin rats more resembled type 1 diabetes with decreased body weight and blood insulin. Rats with 25 mg/kg streptozotocin followed by normal diet feeding showed normalized blood glucose level and pancreatic structure, indicating that normal diet might help recovery from certain symptoms of type 2 diabetes. In comparison, diabetic rats fed with high-fat diet presented decreased but relatively stable blood glucose level, and this was significantly higher than that of the control group (P<0.05). Conclusions: This model easily recovers with normal diet feeding. A high-fat diet is suggested as the background diet in future pharmacological studies using this model.


方法:饲喂Wistar大鼠高脂饮食8周后,注射25或 35 mg/kg STZ来诱导二型糖尿病.之后每组分别饲喂正常饲料和高脂饲料4周,检测血液生化指标的稳定性.
结论:不管在何种饮食下,糖尿病大鼠都出现了空腹血糖显著恢复,血脂紊乱加剧的现象.其中35 mg/kg STZ注射组大鼠的特征更接近一型糖尿病,而 25 mg/kg STZ注射组大鼠表现出二型糖尿病的特征.相比高脂饮食,正常饮食更容易导致空腹血糖和胰岛结构的恢复.因此,在正常饮食下该模型稳定性欠佳,在后期的药理学实验中推荐使用高脂饮食.


Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


[1]Bhattacharya S, Dey D, Roy SS, 2007. Molecular mechanism of insulin resistance. J Biosci, 32(2):405-413.

[2]Bibak B, Khalili M, Rajaei Z, et al., 2014. Effects of melatonin on biochemical factors and food and water consumption in diabetic rats. Adv Biomed Res, 3(1):173.

[3]Garg A, Misra A, 2002. Hepatic steatosis, insulin resistance, and adipose tissue disorders. J Clin Endocrinol Metab, 87(7):3019-3022.

[4]Hotamisligil GS, Shargill NS, Spiegelman BM, 1993. Adipose expression of tumor necrosis factor-alpha: direct role in obesity-linked insulin resistance. Science, 259(5091):87-91.

[5]Institute of Laboratory Animal Resources Committee, 1996. Guide for the Care and Use of Laboratory Animals. National Academy Press, Washington DC.

[6]Ji J, Zhang C, Luo X, et al., 2015. Effect of stay-green wheat, a novel variety of wheat in China, on glucose and lipid metabolism in high-fat diet induced type 2 diabetic rats. Nutrients, 7(7):5143-5155.

[7]Lee JS, Son HS, Maeng YS, et al., 1994. Effects of buckwheat on organ weight, glucose and lipid metabolism in streptozotocin-induced diabetic rats. J Korean Soc Food Sci Nutr, 27(8):819-827.

[8]Leedom LJ, Meehan WP, 1989. The psychoneuroendocrinology of diabetes mellitus in rodents. Psychoneuroendocrinology, 14(4):275-294.

[9]Liu J, Zhang H, Ji B, et al., 2014. A diet formula of Puerariae radix, Lycium barbarum, Crataegus pinnatifida, and Polygonati rhizoma alleviates insulin resistance and hepatic steatosis in CD-1 mice and HepG2 cells. Food Funct, 5(5):1038-1049.

[10]Luo J, Quan J, Tsai J, et al., 1998. Nongenetic mouse models of non-insulin-dependent diabetes mellitus. Metabolism, 47(6):663-668.

[11]Mahmoud AM, Ashour MB, Abdel-Moneim A, et al., 2012. Hesperidin and naringin attenuate hyperglycemiamediated oxidative stress and proinflammatory cytokine production in high fat fed/streptozotocin-induced type 2 diabetic rats. J Diabetes Complications, 26(6):483-490.

[12]Mansor LS, Gonzalez ER, Cole MA, et al., 2013. Cardiac metabolism in a new rat model of type 2 diabetes using high-fat diet with low dose streptozotocin. Cardiov Diabetol, 12(1):136.

[13]Marx JL, 1979. The HDL: the good cholesterol carriers? Science, 205(4407):677-679.

[14]Rathmann W, Giani G, 2004. Global prevalence of diabetes: estimates for the year 2000 and projections for 2030. Diabetes Care, 27(10):2568-2569.

[15]Reed M, Meszaros K, Entes L, et al., 2000. A new rat model of type 2 diabetes: the fat-fed, streptozotocin-treated rat. Metabolism, 49(11):1390-1394.

[16]Rossmeisl M, Rim JS, Koza RA, et al., 2003. Variation in type 2 diabetes-related traits in mouse strains susceptible to diet-induced obesity. Diabetes, 52(8):1958-1966.

[17]Sahin K, Onderci M, Tuzcu M, et al., 2007. Effect of chromium on carbohydrate and lipid metabolism in a rat model of type 2 diabetes mellitus: the fat-fed, streptozotocin-treated rat. Metabolism, 56(9):1233-1240.

[18]Schnedl WJ, Ferber S, Johnson JH, et al., 1994. STZ transport and cytotoxicity: specific enhancement in GLUT2-expressing cells. Diabetes, 43(11):1326-1333.

[19]Shafrir E, 2003. Diabetes in animals: contribution to the understanding of diabetes by study of its etiopathology in animal models. In: Porte D, Sherwin RS, Baron A (Eds.), Diabetes Mellitus. McGraw-Hill, New York.

[20]Shatwan IA, Ahmed LA, Badkook MM, 2013. Effect of barley flour, crude cinnamon, and their combination on glycemia, dyslipidemia, and adipose tissue hormones in type 2 diabetic rats. J Med Food, 16(7):656-662.

[21]Shaw JE, Sicree RA, Zimmet PZ, 2010. Global estimates of the prevalence of diabetes for 2010 and 2030. Diabetes Res Clin Pract, 87(1):4-14.

[22]Siri-Tarino PW, Sun Q, Hu FB, et al., 2010. Saturated fatty acids and risk of coronary heart disease: modulation by replacement nutrients. Curr Atherosclerosis Rep, 12(6): 384-390.

[23]Srinivasan K, Viswanad B, Asrat L, et al., 2005. Combination of high-fat diet-fed and low-dose streptozotocin-treated rat: a model for type 2 diabetes and pharmacological screening. Pharmacol Res, 52(4):313-320.

[24]Tan BKH, Tan CH, Pushparaj PN, 2005. Anti-diabetic activity of the semi-purified fractions of Averrhoa bilimbi in high fat diet fed-streptozotocin-induced diabetic rats. Life Sci, 76(24):2827-2839.

[25]Vinson JA, Zhang J, 2005. Black and green teas equally inhibit diabetic cataracts in a streptozotocin-induced rat model of diabetes. J Agric Food Chem, 53(9):3710-3713.

[26]Wang C, Li J, Lv X, et al., 2009. Ameliorative effect of berberine on endothelial dysfunction in diabetic rats induced by high-fat diet and streptozotocin. Eur J Pharmacol, 620(1-3):131-137.

[27]Wang O, Liu J, Cheng Q, et al., 2015. Effects of ferulic acid and γ-oryzanol on high-fat and high-fructose diet-induced metabolic syndrome in rats. PLoS ONE, 10(2):e0118135.

[28]Wang Y, Campbell T, Perry B, et al., 2011. Hypoglycemic and insulin-sensitizing effects of berberine in high-fat diet- and streptozotocin-induced diabetic rats. Metabolism, 60(2):298-305.

[29]Watts LM, Manchem VP, Leedom TA, et al., 2005. Reduction of hepatic and adipose tissue glucocorticoid receptor expression with antisense oligonucleotides improves hyperglycemia and hyperlipidemia in diabetic rodents without causing systemic glucocorticoid antagonism. Diabetes, 54(6):1846-1853.

[30]Zhang L, Yang J, Chen X, et al., 2010. Antidiabetic and antioxidant effects of extracts from Potentilla discolor Bunge on diabetic rats induced by high fat diet and streptozotocin. J Ethnopharmacol, 132(2):518-524.

Open peer comments: Debate/Discuss/Question/Opinion


Please provide your name, email address and a comment

Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - 2024 Journal of Zhejiang University-SCIENCE