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CLC number: S857.2+6

On-line Access: 2013-01-08

Received: 2012-04-24

Revision Accepted: 2012-11-09

Crosschecked: 2012-12-17

Cited: 14

Clicked: 6300

Citations:  Bibtex RefMan EndNote GB/T7714

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Journal of Zhejiang University SCIENCE B 2013 Vol.14 No.1 P.33-39


Clinicobiochemical investigations of gangrenous mastitis in does: immunological responses and oxidative stress biomarkers

Author(s):  Wael M. El-Deeb

Affiliation(s):  Department of Clinical Studies, College of Veterinary Medicine and Animal Resources, King Faisal University, P.O. Box 400, Al-Ahsa 31982, Saudi Arabia; more

Corresponding email(s):   drwaeleldeeb@yahoo.com

Key Words:  Haptoglobin, Serum amyloid A, Malondialdehyde, Interleukin 1β

Wael M. El-Deeb. Clinicobiochemical investigations of gangrenous mastitis in does: immunological responses and oxidative stress biomarkers[J]. Journal of Zhejiang University Science B, 2013, 14(1): 33-39.

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author="Wael M. El-Deeb",
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%T Clinicobiochemical investigations of gangrenous mastitis in does: immunological responses and oxidative stress biomarkers
%A Wael M. El-Deeb
%J Journal of Zhejiang University SCIENCE B
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%P 33-39
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%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1200123

T1 - Clinicobiochemical investigations of gangrenous mastitis in does: immunological responses and oxidative stress biomarkers
A1 - Wael M. El-Deeb
J0 - Journal of Zhejiang University Science B
VL - 14
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SP - 33
EP - 39
%@ 1673-1581
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PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.B1200123

A total of 50 does were used to determine selected hematological and biochemical parameters with special references to oxidative stress markers, acute phase protein profiles, and proinflammatory cytokines in healthy and gangrenous mastitis affected does. Animals were divided into two equal groups represented as clinically healthy (control) and diseased groups, respectively. The bacteriological examination of milk samples from diseased does revealed many types of bacterial infection. The isolated bacteria were Staphylococcus aureus (N=23/25), Escherichia coli (N=11/25), and Clostridium perfringens (N=4/25). There was a significant increase in the levels of β-hydroxybutyrate, non-esterified free fatty acids, triglyceride, low density lipoprotein cholesterol (LDL-C), aspartate aminotransferase, and alanine aminotransferase and a significant reduction in the levels of glucose, cholesterol, and high density lipoprotein cholesterol (HDL-C) in does with gangrenous mastitis compared to healthy does. Moreover, there was a significant increase in the levels of malondialdehyde and uric acid with a significant decrease in the levels of reduced glutathione, super oxide dismutase, and catalase in does with gangrenous mastitis compared to healthy does. In addition, there was a significant increase in the haptoglobin, serum amyloid A, fibrinogen, interleukin 6 (IL-6), IL-1β, and tumor necrosis factor-α; (TNF-α) in does with gangrenous mastitis compared to healthy ones. Conclusively, oxidative stress biomarkers, acute phase proteins, and proinflammatory cytokines play an essential task as biomarkers for gangrenous mastitis in does. Mastitis may be considered as one of the ketotic stressors in does after parturition.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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Open peer comments: Debate/Discuss/Question/Opinion


Socan G@Professor<No mail>

2013-04-23 12:37:05

It is very interesting paper Thanks for author effort.

Sauzan JK@No address<No mail>

2012-12-11 13:20:56

good work thanks to author effort

Anonymous@No address<No mail>

2012-11-28 17:27:34

It is very interesting paper

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